Table 1.

Key methodologic aspects of selected PDX collections

ReferenceTumor typeAvailable modelsOriginProcurementProcessingMice strainImplantation siteEngraftment rate
(28)CRC130MetastasisSurgeryFresh tumor pieces in MatrigelNOD/SCIDs.c.87%
(29)CRC54Primary (35)SurgeryFresh tumor piecesNudes.c.64%
Metastasis (19)
(76)CRC41PrimarySurgeryFresh tumor piecesNudeOrthotopic89.1%
(34)HBC25PrimarySurgeryFresh tumor piecesNudes.c.13%
(30)HBC12Primary (4)Surgery/fluid drainageFresh tumor pieces in MatrigelNOD/SCID with estrogen supplementation for ER+ tumorsMammary fat pad27%
Metastasis (8)
(16)HBC24PrimaryBiopsies/surgery/fluid drainageFresh tumor piecesSCID/Beige and NSG w/wo estrogen and immortalized human fibro-blastsMammary fat pad3%–21%
(21)NSCLC25PrimarySurgeryFresh tumor piecesNOD/SCIDs.c.25%
(20, 22)NSCLC32PrimarySurgeryFresh tumor piecesNOD/SCIDRenal capsule90%
(33)PDAC42PrimarySurgeryFresh tumor pieces in MatrigelNudes.c.61%
(26)PDAC14PrimarySurgeryFresh tumor pieces in MatrigelNudes.c.NR
(77)PDAC16Primary (11)SurgeryFresh tumor piecesNudeOrthotopic62%
Metastasis (5)
(78)SCCHN22PrimaryBiopsy/surgeryFresh tumor pieces in MatrigelNSGs.c.85%
(25)SCCHN/SCC21PrimarySurgeryFresh tumor pieces in MatrigelNudes.c.54%
FOM/FOT
(14)Uveal Melanoma25Primary (73)SurgeryFresh tumor piecesNOD/SCIDs.c.28%
Metastasis (17)

NOTE: This table provides a summary of the methodologic approaches used to generate the most comprehensive PDX collections currently available.

Abbreviations: CRC, colorectal cancer; FOM, floor of the mouth; FOT, floor of the tongue; HBC, human breast cancer; NR, not reported; PDAC, pancreatic ductal adenocarcinoma; RCC, renal cell cancer; s.c., subcutaneous implantation; SCC, squamous cell carcinoma; SCCHN, squamous cell carcinoma of the head and neck.