Table 3.

Nucleotide binding affinities and intrinsic GTP hydrolysis rates of relevant NRAS isoforms

RAS mutantGDP off (rate, per second × 10−4)GDP off + 1:2 SOScat (rate, per second × 10−4)GMPPNP off (rate, per second × 10−4)GMPPNP off + 1:2 SOScat (rate, per second × 10−4)Intrinsic hydrolysis (rate, per second × 10−4)
NRASWT1.5 ± 0.28.0 ± 0.71.7 ± 0.49.8 ± 0.94.6 ± 0.4
NRASG12D1.3 ± 0.33.3 ± 0.41.6 ± 0.53.9 ± 0.62.3 ± 0.5
NRASQ61R1.1 ± 0.21.3 ± 0.30.4 ± 0.20.5 ± 0.20.002 ± 0.03