Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Journal Sections
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Collections
      • COVID-19 & Cancer Resource Center
      • Precision Medicine and Therapeutic Resistance
      • Clinical Trials
      • Immuno-oncology
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
    • Journal Press Releases
  • COVID-19
  • Webinars
  • 10th Anniversary
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • My Cart

Search

  • Advanced search
Cancer Discovery
Cancer Discovery
  • Home
  • About
    • The Journal
    • AACR Journals
    • Journal Sections
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Collections
      • COVID-19 & Cancer Resource Center
      • Precision Medicine and Therapeutic Resistance
      • Clinical Trials
      • Immuno-oncology
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citation
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
    • Journal Press Releases
  • COVID-19
  • Webinars
  • 10th Anniversary
  • Search More

    Advanced Search

Research Briefs

Janus Kinase 3–Activating Mutations Identified in Natural Killer/T-cell Lymphoma

Ghee Chong Koo, Soo Yong Tan, Tiffany Tang, Song Ling Poon, George E. Allen, Leonard Tan, Soo Ching Chong, Whee Sze Ong, Kevin Tay, Miriam Tao, Richard Quek, Susan Loong, Kheng-Wei Yeoh, Swee Peng Yap, Kuo Ann Lee, Lay Cheng Lim, Daryl Tan, Christopher Goh, Ioana Cutcutache, Willie Yu, Cedric Chuan Young Ng, Vikneswari Rajasegaran, Hong Lee Heng, Anna Gan, Choon Kiat Ong, Steve Rozen, Patrick Tan, Bin Tean Teh and Soon Thye Lim
Ghee Chong Koo
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Soo Yong Tan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tiffany Tang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Song Ling Poon
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
George E. Allen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leonard Tan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Soo Ching Chong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Whee Sze Ong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kevin Tay
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Miriam Tao
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Richard Quek
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susan Loong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kheng-Wei Yeoh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Swee Peng Yap
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kuo Ann Lee
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lay Cheng Lim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daryl Tan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher Goh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ioana Cutcutache
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Willie Yu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cedric Chuan Young Ng
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vikneswari Rajasegaran
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hong Lee Heng
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna Gan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Choon Kiat Ong
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Steve Rozen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrick Tan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bin Tean Teh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Soon Thye Lim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/2159-8290.CD-12-0028 Published July 2012
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

The molecular pathogenesis of natural killer/T-cell lymphoma (NKTCL) is not well understood. We conducted whole-exome sequencing and identified Janus kinase 3 (JAK3) somatic–activating mutations (A572V and A573V) in 2 of 4 patients with NKTCLs. Further validation of the prevalence of JAK3 mutations was determined by Sanger sequencing and high-resolution melt (HRM) analysis in an additional 61 cases. In total, 23 of 65 (35.4%) cases harbored JAK3 mutations. Functional characterization of the JAK3 mutations support its involvement in cytokine-independent JAK/STAT constitutive activation leading to increased cell growth. Moreover, treatment of both JAK3-mutant and wild-type NKTCL cell lines with a novel pan-JAK inhibitor, CP-690550, resulted in dose-dependent reduction of phosphorylated STAT5, reduced cell viability, and increased apoptosis. Hence, targeting the deregulated JAK/STAT pathway could be a promising therapy for patients with NKTCLs.

Significance: Gene mutations causing NKTCL have not been fully identified. Through exome sequencing, we identified activating mutations of JAK3 that may play a significant role in the pathogenesis of NKTCLs. Our findings have important implications for the management of patients with NKTCLs. Cancer Discov; 2(7); 591–7. ©2012 AACR.

This article is highlighted in the In This Issue feature, p. 569.

Introduction

Natural killer/T-cell lymphoma (NKTCL) is particularly prevalent in Asian countries and some parts of Latin America. It accounts for up to half of all mature TCL cases in Asia (1). However, compared with the more common B-cell lymphomas, very little is known about its molecular characteristics and pathogenesis. There has been little progress in basic science and clinical research in this subtype of lymphoma, which continues to constitute a major challenge in managing these patients as there is currently no accepted standard first-line treatment for NKTCLs. Despite multiagent chemotherapy and involved-field radiotherapy, the 5-year overall survival rate is approximately 9% for non-nasal NKTCLs and 42% for nasal NKTCLs (2, 3). There is thus an urgent need to identify novel genetic aberrations and potential treatment targets in NKTCLs.

In this study, we conducted whole-exome sequencing to identify somatic mutations in protein-coding genes of NKTCL tumors to shed light on their pathogenesis and to uncover potential new therapeutic targets, which are urgently needed.

Results

Identification and Validation of JAK3 Mutations

Whole-exome sequencing was successfully conducted on fresh-frozen NKTCLs and paired blood samples from 4 different patients. The average coverage of each base in the targeted regions was 111-fold; 84% of the bases were represented at least 20 times (Supplementary Table S1) and a total of 208 somatic mutations were identified in 201 genes (Supplementary Table S2). Known somatic mutations in NKTCLs, such as TP53, KRAS, and NRAS (4), identified by exome sequencing were further validated by Sanger sequencing in the same tumors (Supplementary Table S2). Interestingly, several somatic heterozygous Janus kinase (JAK) mutations were found in 2 separate samples. One tumor harbored both JAK1Y652D and JAK3A572V mutations, whereas the other tumor harbored a JAK3A573V mutation. The JAK3A572V and JAK3A573V mutations were located at exon 12, in the Janus homology domain 2 (JH2) that negatively regulates the Janus homology domain 1 (JH1) kinase activity (Fig. 1). The JAK1Y652D mutation was located in the JH2 domain as well. All 3 missense mutations were predicted by PolyPhen to be damaging (5).

Figure 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 1.

Identification and characterization of JAK3-activating mutations. Domain structure of JAK3 (bottom) and the positions of JAK3A572V(c.1715C>T) and JAK3A573V (c.1718C>T; top) identified through Sanger sequencing of NKTCL samples.

To determine the prevalence of JAK1 and JAK3 mutations in NKTCLs, we Sanger sequenced an additional 61 NKTCL formalin-fixed, paraffin-embedded (FFPE) cases. In total, we found mutations in JAK3 in 23 of 65 (35.4%) cases (Supplementary Table S3) and for JAK1, besides the case with concomitant JAK1Y652D and JAK3A572V mutations described above, no additional mutations were identified. Among the patients with JAK3 mutations, there were 17 heterozygous JAK3A572V, 2 homozygous JAK3A572V, 2 heterozygous JAK3A573V, 1 homozygous JAK3A573V, and 1 heterozygous with both JAK3A572V and JAK3A573V mutations. These results were further confirmed by high-resolution melt (HRM) analysis (Supplementary Fig. S1). The presence of nonmalignant stroma (our samples contained at least 50% tumor content) or tumor subclones makes it difficult to assess whether these “heterozygous” tumors might actually represent a mixture of JAK3 homozygous–mutated cancer cells admixed with normal tissue. As such, it is possible that the number of homozygous tumors reported is actually an underestimate and that this value should be regarded as a lower limit.

We also conducted Epstein-Barr virus–encoded RNA (EBER) testing on all cases. Apart from 4 older cases that cannot be interpreted, all but one case were positive for EBER, regardless of JAK3 mutation status. The single EBER-negative case was a cutaneous deposit taken from a patient with EBER+ nasal NKTCLs (Supplementary Table S3). In parallel, 50% of extra-nasal cases possessed JAK3 mutations and 31.7% of nasal cases had JAK3 mutations. This latter difference was not statistically significant (Supplementary Table S3).

JAK3A572V-Activating Mutations Confer Cytokine-Independent Growth

Interleukin (IL)-2 is an essential cytokine required for the proliferation and activation of NK cells (6). JAK1 and JAK3 mediate IL-2 receptor signaling through phosphorylation of STAT transcription factors (7). In line with the functional importance of the activating JAK3 mutations identified, we tested whether JAK3 mutations could confer IL-2–independent growth to the NKTCL cell line (NK-S1) that harbors a homozygous JAK3A572V mutation. JAK-mutant (NK-S1) cells showed IL-2–independent growth (Fig. 2A) and constitutive phosphorylation of both JAK3 and STAT5 (Fig. 2B). In contrast, JAK3 wild-type KHYG-1 cells were clearly IL-2–dependent (Fig. 2C and D). Importantly, NK-S1 cells treated with JAK3 siRNAs exhibited a significant reduction in cell proliferation and also decreased autophosphorylation of JAK3 and STAT5, compared with cells treated with control siRNAs (Fig. 3A). Reciprocally, KHYG-1 cells transiently overexpressing a mutated JAK3 (JAK3A572V) cDNA showed IL-2–independent proliferation and autophosphorylation of JAK3 and STAT5 (Fig. 3B). These results indicate that the JAK3-activating mutations are gain-of-function alleles and contribute to the constitutive activity of the JAK/STAT pathway in an IL-2–independent manner.

Figure 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 2.

IL-2–independent growth and constitutive JAK3 and STAT5 phosphorylation in a JAK3A572V-mutant NKTCL cell line. NK-S1 (JAK3A572V homozygous mutant) and KHYG-1 (wild-type JAK3) cells were cultured with or without recombinant human IL-2 up to 7 days and followed by MTS assay. A, the NK-S1 cells harboring JAK3A572V were able to grow in an IL-2–independent manner. B, NK-S1 cell lysates were harvested for Western blotting, and the results showed that phosphorylation of JAK3 and STAT5 are IL-2–independent. C, KHYG-1 cells carrying wild-type JAK3 showed IL-2–dependent proliferation. D, phosphorylation of JAK3 and STAT5 in KHYG-1 cells were IL-2–dependent. Experiments were repeated at least 3 times.y

Figure 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 3.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. A, NK-S1 cells were treated with 100 nmol/L JAK3 siRNA (si-JAK3) or control siRNA (si-Ctrl) for 24 hours before being subjected to proliferation assays up to 72 hours (right). In parallel, these cells were harvested, and protein extracts were subjected to Western blotting with antibodies against phosphorylated JAK3 (p-JAK3), phosphorylated STAT5 (p-STAT5), JAK3, STAT5, or β-actin as a normalization control. B, KHYG-1 cells were transiently transfected with wild-type JAK3 (JAK3 WT) or mutated JAK3 expression vectors (i.e., JAK3A572V). The relative p-JAK3, p-STAT5, JAK3, and STAT5 levels in these cells were detected by Western blotting (top), and proliferation assays using these cells were conducted for 48 hours with or without IL-2 (bottom). All results are expressed as mean ± SEM of 3 independent experiments. *, P < 0.05 compared with vehicle control (vehicle).

Effects of CP-690550 on NKTCL Cell Lines

To further confirm the involvement of JAK/STAT signaling in the survival of NKTCLs, we next evaluated the effect of a pan-JAK inhibitor, CP-690550, in NK-S1, KHYG-1, and K562 cells. As expected, both the NK-S1 and KHYG-1 cells showed a reduction of phosphorylated STAT5 (Fig. 4A) and cell viability in a dose-dependent fashion (Fig. 4B). Furthermore, Annexin V staining revealed that the reduction of NK-S1 viability was due to an increase in cellular apoptosis (Fig. 4C). However, this phenomenon was not observed in the K562 cells in which STAT5 phosphorylation is dependent on BCR/ABL1 (8) and not JAK3 (Fig. 4A and B).

Figure 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Figure 4.

Effects of CP-690550 on NKTCL cell lines. A, NK-S1, KHYG-1, and K562 cells were treated with CP-690550 for 48 hours, and the effect on STAT5 phosphorylation was evaluated by Western blotting. B, cell viability was analyzed by MTS assay after the cells were treated with their respective treatment for 72 hours. C, drug-induced apoptosis was evaluated by Annexin V-FITC (fluorescein isothiocyanate) staining, followed by flow cytometric analysis. Both NK-S1 and KHYG-1 cells showed a dose-dependent reduction in STAT5 phosphorylation. Treatment with CP-690550 resulted in reduced cell viability of NK-S1 and KHYG-1 cells but not in K562 cells. Experiments were repeated at least 3 times. Data were analyzed by paired t test, and values significantly different from control are shown as *, P< 0.05; **, P< 0.005; ***, P< 0.001.

Discussion

The JAK/STAT pathway is a key component in normal hematopoiesis. The JAK family of tyrosine kinases comprises 4 members: JAK1, JAK2, JAK3, and TYK2. Among these 4 members, JAK3 signaling is specifically related to T-cell development and proliferation (8) with loss-of-function mutations resulting in severe combined immunodeficiency characterized by the lack of T and NK cells (9). Recent data suggest that mutations resulting in persistent activation of JAK/STAT signaling are involved in the pathogenesis of T-cell acute lymphoblastic lymphoma/leukemia, cutaneous TCL, mantle cell lymphoma, acute megakaryoblastic leukemia, and myeloproliferative diseases (8, 10–16).

Besides hematologic neoplasia, nonhematologic cancers, including breast, stomach, and lung cancer, have also been found to harbor JAK3 mutations (17, 18). To date, transforming ability of the activating mutations of JAK3 (such as P132T, L156P, R172Q, E183G, Q501H, M511I, A572V, A573V, R657Q, and V722I) has been previously validated in Ba/F3 cells (8, 12, 19, 20). In line with these observations, we identified the presence of activating JAK3 mutations in 35% of NKTCL tumors. The JAK3A572V and JAK3A573V mutations found in our samples were located at the JH2 pseudokinase domain that is known to have an autoinhibitory effect on the JH1 kinase domain. Cellular studies revealed that the NK-S1 cells harboring the homozygous JAK3A572V mutation are able to proliferate in cell culture without IL-2 stimulation, with constitutive expression of both phosphorylated JAK3 and STAT5. Cornejo and colleagues (21) showed that when JAK3A572V retroviral–transduced bone marrow cells were transplanted into C57BL/6 and BALB/c mice, there was a constitutive activation of JAK/STAT signaling which led to the development of fatal polyclonal T-cell lymphoproliferative disorder. In accordance, transiently overexpressing a JAK3A572V in a JAK3 wild-type NKTCL cell line (KHYG-1) resulted in IL-2–independent cell proliferation and the activation of JAK/STAT signaling pathways. Thus, it is conceivable that the JAK3 mutation may play an important role in the pathogenesis of NKTCLs.

CP-690550, a novel pan-JAK inhibitor, has recently been shown to inhibit adult TCL/leukemia (ATLL) cells (22) and ATLL xenograft tumors and is currently in phase III trials for the treatment of rheumatoid arthritis (23). Consistent with the high frequency of JAK3 mutations (35%) in NKTCLs, use of CP-690550 in the JAK3-mutant NKTCL cell line showed inhibition in the phosphorylation of STAT5 along with reduced cell viability. These data are compelling and suggest a potential target for this otherwise fatal disease.

In summary, our studies identified, for the first time, frequent JAK3 mutations in NKTCLs. They also indicated that targeting the JAK/STAT pathway in this disease is a potentially effective therapeutic approach that warrants further investigation.

Methods

Tissue Samples

Matched fresh-frozen tissue and peripheral blood samples were obtained from 4 consented patients with NKTCLs for whole-exome sequencing. The JAK3, JAK1, JAK2, and TYK2 mutation status in these samples was confirmed by Sanger sequencing of all coding exons. FFPE tissue blocks from 61 patients with NKTCLs were procured for mutation analysis. The diagnosis of NKTCL was made according to the 2008 World Health Organization classification of tumors of the hematopoietic and lymphoid tissues (24). All samples were centrally reviewed by our hematopathologists. This study was approved by the SingHealth Centralized Institutional Review Board (CIRB), study number 2004/407/B.

Preparation of Genomic DNA

DNA of frozen tissue and paired blood samples was isolated using a DNeasy blood and tissue mini kit and a QIAmp DNA blood midi kit (Qiagen), respectively, according to the manufacturer’s instruction. For FFPE samples, genomic DNA was extracted from one or two 10-μm slices from each sample by removal of paraffin followed by proteinase K digestion according to standard procedures. DNA was then extracted using a DNeasy blood and tissue mini kit (Qiagen).

Whole-Exome Sequencing and Identification of Candidate Mutations

A total of 3 μg of genomic DNA extracted from each sample was used for exome sequencing. Captured DNA libraries were sequenced with the Illumina GAIIx Genome Analyzer, yielding 150 (2 × 75) base pairs from the final library fragments. We used Burrows Wheeler Aligner to align the sequence reads to the human reference genome NCBI built 37.1 (hg19) and then we ran SamTools to remove PCR duplicates. To detect single-nucleotide variants (SNV), we used a discovery pipeline based on the Genome Analyzer Toolkit (GATK). Our pipeline first recalibrates the base qualities and realigns the sequence reads around micro-indels. The next step uses a GATK Unified Genotyper that does the consensus calling to identify SNVs. Only well-mapped reads (mapping quality ≥30, number of mismatches within a 40-bp window ≤3) were used as input to the genotyper. We retained SNVs that passed additional quality filters (a quality by depth ≥5, a variant depth ≥5, a normal depth ≥5) and discarded any SNV close to a micro-indel or to several other SNVs. We compared our variants against the common polymorphisms present in dbSNP 131 and in the 1000 genomes databases to discard any common SNPs. All variants retained following this step were considered to be novel. Several gene transcript annotation databases (CCDS, RefSeq, Ensembl, and UCSC) were used for transcript identification and for determining the amino acid change. Only SNVs in exons or in canonical splice sites were further analyzed. Amino acid changes corresponding to SNVs were annotated according to the largest transcript of the gene.

Mutation Validation by HRM and Sanger Sequencing

Sanger sequencing and HRM (25, 26) were used to confirm the JAK3 and JAK1 mutations identified and validate their prevalence in our NKTCL patient population. The primer sequences used for validation are listed in Supplementary Table S4. For Sanger sequencing, PCR was carried out with Platinum Taq Polymerase (Life Technologies, catalog number 10966-083) and cycled at 95°C for 10 minutes; 39 cycles of 95°C for 30 seconds; 60°C for 30 seconds, 72°C for 1 minute, and a final extension of 72°C for 10 minutes. Sequencing PCR was carried out with ABI BigDye Terminator v3.1 (Life Technologies, catalog number 4337457) and cycled at 96°C for 1 minute; 29 cycles of 96°C for 10 seconds; 50°C for 5 seconds, and 60°C for 4 minutes. The resulting products were run on an ABI 3730 DNA analyzer. For HRM analysis, SsoFast EvaGreen Supermix (Bio-Rad, catalog number 172-5200) was used in the amplification of the sample amplicons using the JAK3 HRM primers at a final concentration of 600 nmol/L on a Bio-Rad CFX96 Real-Time PCR detection system in replicates. The cycling and melting conditions were as follows: 1 cycle of 98°C for 2 minutes; 39 cycles of 98°C for 5 minutes; 58°C for 10 minutes; 1 cycle of 95°C for 30 minutes; and a melt from 72°C to 95°C increasing at 0.2°C/s. The HRM curves were analyzed with the Bio-Rad Precision Melt Analysis Software. HRM difference curves deviating from the wild-type curve were considered to be harboring a mutation.

Cell Lines

NK-S1, established from a previously described NKTCL xenograft (27), was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Life Technologies) supplemented with 10% heat-inactivated FBS and 10% equine serum. KHYG-1 was obtained from the Japanese Collection of Research BioResources (28) and cultured in RPMI medium (Life Technologies) supplemented with heat-inactivated FBS (10%), equine serum (10%), and 200 IU/mL of recombinant human IL-2 (Novartis). K562 (CCL-234) was purchased from American Type Culture Collection and cultured in DMEM supplemented with 10% heat-inactivated FBS and 10% equine serum. The coding exons of JAK3 were fully sequenced in these 3 cell lines, and we confirmed that only NK-S1 harbored a homozygous JAK3A572V mutation.

Cell Line Transfections

JAK3 siRNA or control siRNA (Dharmacon) were transfected into NK-S1 cell line using RNAiMAX (Invitrogen) according to the manufacturer’s protocols. MIGR1 expression vectors containing full-length wild-type JAK3 or JAK3A572V mutant were generously provided by Dr. Brian Druker (Howard Hughes Medical Institute, Chevy Chase, MD; ref. 8). Transient overexpression of these 2 constructs in KHGY-1 cells was then generated using Effectene Transfection Reagent (Qiagen Inc.).

Cell Viability and Apoptosis Assays

Cells were seeded at 2 × 104 cells/100 μL/well in 96-well plates and treated with or without CP-690550 (Selleck Chemical, S5001) at various concentrations before being subjected to MTS assay (Promega). The extent of drug-induced apoptosis was evaluated by Annexin V-FITC (BD Biosciences) staining. Acquisition of the data were conducted on FACSCalibur flow cytometer (BD Biosciences).

Western Blot

Cells were harvested at indicated time intervals after IL-2 or CP-690550 treatment. Total proteins were extracted with lysis buffer, resolved by SDS-PAGE gels, and blotted onto a nitrocellulose membrane. After blocking, membranes were probed with primary antibodies against phospho-STAT5 (Cell Signaling, catalog number 9356), STAT5 (Cell Signaling, catalog number 9363), phospho-JAK3 (Cell Signaling, catalog number 5031), JAK3 (Cell Signaling, catalog number 3775), and β-actin followed by either peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody. Signals were visualized with enhanced chemiluminescence (ECL; Amersham).

Disclosure of Potential Conflicts of Interest

S. Rozen is employed by Duke University as Associate Professor Track V. No potential conflicts of interests were disclosed by the other authors.

Authors’ Contributions

Conception and design: G.C. Koo, S.Y. Tan, K. Tay, B.T. Teh, S.T. Lim

Development of methodology: G.C. Koo, K.-W. Yeoh, C.C.Y. Ng, V. Rajasegaran, S. Rozen, B.T. Teh, S.T. Lim

Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc.): G.C. Koo, S.Y. Tan, L. Tan, S.C. Chong, K. Tay, M. Tao, R. Quek, S. Loong, K.-W. Yeoh, S.P. Yap, K.A. Lee, L.C. Lim, D. Tan, C. Goh, C.C.Y. Ng, V. Rajasegaran, H.L. Heng, A. Gan, S.T. Lim

Analysis and interpretation of data (e.g., statistical analysis, biostatistics, computational analysis): G.C. Koo, S.Y. Tan, S.L. Poon, G.E. Allen, W.S. Ong, K.-W. Yeoh, K.A. Lee, I. Cutcutache, W. Yu, C.C.Y. Ng, V. Rajasegaran, H.L. Heng, A. Gan, C.K. Ong, S. Rozen, P. Tan, B.T. Teh, S.T. Lim

Writing, review, and/or revision of the manuscript: G.C. Koo, S.Y. Tan, T. Tang, S.L. Poon, W.S. Ong, K. Tay, R. Quek, K.-W. Yeoh, L.C. Lim, C. Goh, W. Yu, C.C.Y. Ng, C.K. Ong, P. Tan, B.T. Teh, S.T. Lim

Administrative, technical, or material support (i.e., reporting or organizing data, constructing databases): S.L. Poon, K. Tay, C.C.Y. Ng, S.T. Lim

Study supervision: S. Rozen, B.T. Teh, S.T. Lim

Supplied pathologic diagnosis for case series: L. Tan

Grant Support

This study was funded by the National Medical Research Council of Singapore (NMRC/PPG/NCC/2011) as well as a grant from HSBC Trustee (Singapore) Limited as trustees of the Major John Long Trust Fund and the Chew Woon Poh Trust Fund.

Acknowledgments

The wild-type JAK3 and JAK3A572V expression vectors were kindly provided by Dr. Brian Druker. The authors also thank the Lee Foundation for its support, Huang Dachuan and Waraporn Chan-on for proofreading the manuscript, and Sabrina Noyes for assistance in manuscript submission. This study is dedicated to Dr. Han Mo Koo.

Footnotes

  • Note: Supplementary data for this article are available at Cancer Discovery Online (http://cancerdiscovery.aacrjournals.org/).

  • Received January 27, 2012.
  • Revision received May 7, 2012.
  • Accepted May 8, 2012.
  • ©2012 American Association for Cancer Research.

References

  1. 1.↵
    1. Kwong YL,
    2. Anderson BO,
    3. Advani R,
    4. Kim WS,
    5. Levine AM,
    6. Lim ST.
    Management of T-cell and natural-killer-cell neoplasms in Asia: consensus statement from the Asian Oncology Summit 2009. Lancet Oncol 2009;10:1093–101.
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Au WY,
    2. Ma SY,
    3. Chim CS,
    4. Choy C,
    5. Loong F,
    6. Lie AK,
    7. et al.
    Clinicopathologic features and treatment outcome of mature T-cell and natural killer-cell lymphomas diagnosed according to the World Health Organization classification scheme: a single center experience of 10 years. Ann Oncol 2005;16:206–14.
    OpenUrlAbstract/FREE Full Text
  3. 3.↵
    1. Vose J,
    2. Armitage J,
    3. Weisenburger D.
    International peripheral T-cell and natural killer/T-cell lymphoma study: pathology findings and clinical outcomes. J Clin Oncol 2008;26:4124–30.
    OpenUrlAbstract/FREE Full Text
  4. 4.↵
    1. Takahara M,
    2. Kishibe K,
    3. Bandoh N,
    4. Nonaka S,
    5. Harabuchi Y.
    P53, N- and K-Ras, and beta-catenin gene mutations and prognostic factors in nasal NK/T-cell lymphoma from Hokkaido, Japan. Hum Pathol 2004;35:86–95.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Sunyaev S,
    2. Ramensky V,
    3. Koch I,
    4. Lathe W III,
    5. Kondrashov AS,
    6. Bork P.
    Prediction of deleterious human alleles. Hum Mol Genet 2001;10:591–7.
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Suzuki R,
    2. Handa K,
    3. Itoh K,
    4. Kumagai K.
    Natural killer (NK) cells as a responder to interleukin 2 (IL 2). I. Proliferative response and establishment of cloned cells.. J Immunol 1983;130:981–7.
    OpenUrlAbstract/FREE Full Text
  7. 7.↵
    1. Lu L,
    2. Zhu J,
    3. Zheng Z,
    4. Yan M,
    5. Xu W,
    6. Sun L,
    7. et al.
    Jak-STAT pathway is involved in the induction of TNF-beta gene during stimulation by IL-2. Eur J Immunol 1998;28:805–10.
    OpenUrlCrossRefPubMed
  8. 8.↵
    1. Walters DK,
    2. Mercher T,
    3. Gu TL,
    4. O’Hare T,
    5. Tyner JW,
    6. Loriaux M,
    7. et al.
    Activating alleles of JAK3 in acute megakaryoblastic leukemia. Cancer Cell 2006;10:65–75.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Notarangelo LD,
    2. Mella P,
    3. Jones A,
    4. de Saint Basile G,
    5. Savoldi G,
    6. Cranston T,
    7. et al.
    Mutations in severe combined immune deficiency (SCID) due to JAK3 deficiency. Hum Mutat 2001;18:255–63.
    OpenUrlCrossRefPubMed
  10. 10.
    1. Flex E,
    2. Petrangeli V,
    3. Stella L,
    4. Chiaretti S,
    5. Hornakova T,
    6. Knoops L,
    7. et al.
    Somatically acquired JAK1 mutations in adult acute lympho-blastic leukemia. J Exp Med 2008;205:751–8.
    OpenUrlAbstract/FREE Full Text
  11. 11.
    1. Bercovich D,
    2. Ganmore I,
    3. Scott LM,
    4. Wainreb G,
    5. Birger Y,
    6. Elimelech A,
    7. et al.
    Mutations of JAK2 in acute lymphoblastic leukaemias associated with Down’s syndrome. Lancet 2008;372:1484–92.
    OpenUrlCrossRefPubMed
  12. 12.
    1. Elliott NE,
    2. Cleveland SM,
    3. Grann V,
    4. Janik J,
    5. Waldmann TA,
    6. Dave UP.
    FERM domain mutations induce gain of function in JAK3 in adult T-cell leukemia/lymphoma. Blood 2011;118:3911–21.
    OpenUrlAbstract/FREE Full Text
  13. 13.
    1. Krejsgaard T,
    2. Vetter-Kauczok CS,
    3. Woetmann A,
    4. Lovato P,
    5. Labuda T,
    6. Eriksen KW,
    7. et al.
    Jak3- and JNK-dependent vascular endothelial growth factor expression in cutaneous T-cell lymphoma. Leukemia 2006;20:1759–66.
    OpenUrlCrossRefPubMed
  14. 14.
    1. Yared MA,
    2. Khoury JD,
    3. Medeiros LJ,
    4. Rassidakis GZ,
    5. Lai R.
    Activation status of the JAK/STAT3 pathway in mantle cell lymphoma. Arch Pathol Lab Med 2005;129:990–6.
    OpenUrlPubMed
  15. 15.
    1. Kiyoi H,
    2. Yamaji S,
    3. Kojima S,
    4. Naoe T.
    JAK3 mutations occur in acute megakaryoblastic leukemia both in Down syndrome children and non-Down syndrome adults. Leukemia 2007;21:574–6.
    OpenUrlCrossRefPubMed
  16. 16.
    1. James C,
    2. Ugo V,
    3. Le Couedic JP,
    4. Staerk J,
    5. Delhommeau F,
    6. Lacout C,
    7. et al.
    A unique clonal JAK2 mutation leading to constitutive signalling causes polycythaemia vera. Nature 2005;434:1144–8.
    OpenUrlCrossRefPubMed
  17. 17.↵
    1. Jeong EG,
    2. Kim MS,
    3. Nam HK,
    4. Min CK,
    5. Lee S,
    6. Chung YJ,
    7. et al.
    Somatic mutations of JAK1 and JAK3 in acute leukemias and solid cancers. Clin Cancer Res 2008;14:3716–21.
    OpenUrlAbstract/FREE Full Text
  18. 18.↵
    1. Ding L,
    2. Getz G,
    3. Wheeler DA,
    4. Mardis ER,
    5. McLellan MD,
    6. Cibulskis K,
    7. et al.
    Somatic mutations affect key pathways in lung adenocarcinoma. Nature 2008;455:1069–75.
    OpenUrlCrossRefPubMed
  19. 19.
    1. Malinge S,
    2. Ragu C,
    3. Della-Valle V,
    4. Pisani D,
    5. Constantinescu SN,
    6. Perez C,
    7. et al.
    Activating mutations in human acute megakaryoblastic leukemia. Blood 2008;112:4220–6.
    OpenUrlAbstract/FREE Full Text
  20. 20.
    1. Yamashita Y,
    2. Yuan J,
    3. Suetake I,
    4. Suzuki H,
    5. Ishikawa Y,
    6. Choi YL,
    7. et al.
    Array-based genomic resequencing of human leukemia. Oncogene 2010;29:3723–31.
    OpenUrlCrossRefPubMed
  21. 21.↵
    1. Cornejo MG,
    2. Kharas MG,
    3. Werneck MB,
    4. Le Bras S,
    5. Moore SA,
    6. Ball B,
    7. et al.
    Constitutive JAK3 activation induces lymphoproliferative syndromes in murine bone marrow transplantation models. Blood 2009;113:2746–54.
    OpenUrlAbstract/FREE Full Text
  22. 22.↵
    1. Manshouri T,
    2. Quintas-Cardama A,
    3. Nussenzveig RH,
    4. Gaikwad A,
    5. Estrov Z,
    6. Prchal J,
    7. et al.
    The JAK kinase inhibitor CP-690,550 suppresses the growth of human polycythemia vera cells carrying the JAK2V617F mutation. Cancer Sci 2008;99:1265–73.
    OpenUrlCrossRefPubMed
  23. 23.↵
    1. Ju W,
    2. Zhang M,
    3. Jiang JK,
    4. Thomas CJ,
    5. Oh U,
    6. Bryant BR,
    7. et al.
    CP-690,550, a therapeutic agent, inhibits cytokine-mediated Jak3 activation and proliferation of T cells from patients with ATL and HAM/TSP. Blood 2011;117:1938–46.
    OpenUrlAbstract/FREE Full Text
  24. 24.↵
    1. Campo E,
    2. Swerdlow SH,
    3. Harris NL,
    4. Pileri S,
    5. Stein H,
    6. Jaffe ES.
    The 2008 WHO classification of lymphoid neoplasms and beyond: evolving concepts and practical applications. Blood 2008;117:5019–32.
    OpenUrl
  25. 25.↵
    1. Marchetti A,
    2. Felicioni L,
    3. Buttitta F.
    Assessing EGFR mutations. N Engl J Med 2006;354:526–8; author reply 526–8.
    OpenUrlCrossRefPubMed
  26. 26.↵
    1. Solassol J,
    2. Ramos J,
    3. Crapez E,
    4. Saifi M,
    5. Mange A,
    6. Vianes E,
    7. et al.
    KRAS mutation detection in paired frozen and formalin-fixed paraffin-embedded (FFPE) colorectal cancer tissues. Int J Mol Sci 2011;12:3191–204.
    OpenUrlCrossRefPubMed
  27. 27.↵
    1. Loong SL,
    2. Hwang JS,
    3. Lim ST,
    4. Yap SP,
    5. Tao M,
    6. Chong TW,
    7. et al.
    An Epstein-Barr virus positive natural killer lymphoma xenograft derived for drug testing. Leuk Lymphoma 2008;49:1161–7.
    OpenUrlCrossRefPubMed
  28. 28.↵
    1. Yagita M,
    2. Huang CL,
    3. Umehara H,
    4. Matsuo Y,
    5. Tabata R,
    6. Miyake M,
    7. et al.
    A novel natural killer cell line (KHYG-1) from a patient with aggressive natural killer cell leukemia carrying a p53 point mutation. Leukemia 2000;14:922–30.
    OpenUrlCrossRefPubMed
PreviousNext
Back to top
Cancer Discovery: 2 (7)
July 2012
Volume 2, Issue 7
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover

Sign up for alerts

View this article with LENS

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Discovery article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Janus Kinase 3–Activating Mutations Identified in Natural Killer/T-cell Lymphoma
(Your Name) has forwarded a page to you from Cancer Discovery
(Your Name) thought you would be interested in this article in Cancer Discovery.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Janus Kinase 3–Activating Mutations Identified in Natural Killer/T-cell Lymphoma
Ghee Chong Koo, Soo Yong Tan, Tiffany Tang, Song Ling Poon, George E. Allen, Leonard Tan, Soo Ching Chong, Whee Sze Ong, Kevin Tay, Miriam Tao, Richard Quek, Susan Loong, Kheng-Wei Yeoh, Swee Peng Yap, Kuo Ann Lee, Lay Cheng Lim, Daryl Tan, Christopher Goh, Ioana Cutcutache, Willie Yu, Cedric Chuan Young Ng, Vikneswari Rajasegaran, Hong Lee Heng, Anna Gan, Choon Kiat Ong, Steve Rozen, Patrick Tan, Bin Tean Teh and Soon Thye Lim
Cancer Discov July 1 2012 (2) (7) 591-597; DOI: 10.1158/2159-8290.CD-12-0028

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Janus Kinase 3–Activating Mutations Identified in Natural Killer/T-cell Lymphoma
Ghee Chong Koo, Soo Yong Tan, Tiffany Tang, Song Ling Poon, George E. Allen, Leonard Tan, Soo Ching Chong, Whee Sze Ong, Kevin Tay, Miriam Tao, Richard Quek, Susan Loong, Kheng-Wei Yeoh, Swee Peng Yap, Kuo Ann Lee, Lay Cheng Lim, Daryl Tan, Christopher Goh, Ioana Cutcutache, Willie Yu, Cedric Chuan Young Ng, Vikneswari Rajasegaran, Hong Lee Heng, Anna Gan, Choon Kiat Ong, Steve Rozen, Patrick Tan, Bin Tean Teh and Soon Thye Lim
Cancer Discov July 1 2012 (2) (7) 591-597; DOI: 10.1158/2159-8290.CD-12-0028
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Results
    • Discussion
    • Methods
    • Disclosure of Potential Conflicts of Interest
    • Authors’ Contributions
    • Grant Support
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Fas Mediates Bystander Tumor Killing by T Cells
  • Immune Checkpoint Inhibitor Myocarditis in Mice
  • Genetic Ancestry Impacts Somatic Alterations in Lung Cancers
Show more Research Briefs
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook   Twitter   LinkedIn   YouTube   RSS

Articles

  • OnlineFirst
  • Current Issue
  • Past Issues

Info For

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Discovery

  • About the Journal
  • Editors
  • Journal Sections
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Discovery
eISSN: 2159-8290
ISSN: 2159-8274

Advertisement