Table 1.

FISH analysis of MET and KRAS gene copy for the primary tumor and metastatic deposit at recurrence in the nonsignet ring and signet ring cells

I Primary tumor, nonsignet ring morphology
 MET
  MET/cell: 3.7
  CEP7/cell: 3.53
  MET copy number ≥4: 60%
  Ratio MET/CEP7: 1.04
  Ratio MET/CEP7 ≥2: 0%
  Most common cell type is 4 MET: 4 CEP7 found in 41.67% of nuclei.
 KRAS
  KRAS/cell: 2.27
  CEP12/cell: 2.17
  KRAS copy number ≥4: 0%
  Ratio KRAS/CEP12: 1.05
  Ratio KRAS/CEP12 ≥2: 0%
  Most common cell type is 2 KRAS: 2 CEP12 found in 46.66% of nuclei.
II Transverse colon metastasis, nonsignet ring morphology
 MET
  MET/cell: 3.18
  CEP7/cell: 2.99
  MET copy number ≥4: 42%
  MET/CEP7 ratio: 1.06
  Ratio MET/CEP7 ≥2: 0%
  Most common cell type is 2 MET:2 CEP7 found in 28% of nuclei.
  Second most common is 4 MET:4 CEP7 found in 22% of nuclei.
 KRAS
  KRAS/cell: 2.52
  CEP12/cell: 2.53
  KRAS copy number ≥4: 2%
  Ratio KRAS/CEP12: 1.00
  Ratio KRAS/CEP12 ≥2: 0%
  Most common cell type is 3 KRAS: 3 CEP12 found in 59.00% of nuclei.
III Primary tumor, signet ring morphology
 MET
  MET/cell: 3.7
  CEP7/cell: 3.83
  MET copy number ≥4: 63.33%
  Ratio MET/CEP7: 0.99
  Ratio MET/CEP7 ≥2: 0%
  Most common cell type is 4 MET: 4 CEP7 found in 40% of nuclei.
 KRAS
  KRAS/cell: 2.60
  CEP12/cell: 2.43
  KRAS copy number ≥4: 5.00%
  Ratio KRAS/CEP12: 1.07
  Ratio KRAS/CEP12 ≥2: 0%
  Most common cell type is 3 KRAS:3 CEP12 found in 40.00% of nuclei.
IV Transverse colon metastasis, signet ring morphology
 MET
  MET/cell: 2.93
  CEP7/cell: 2.76
  MET copy number ≥ 4: 36.66%
  MET/CEP7 ratio: 1.06
  Ratio MET/CEP7 ≥ 2: 5.00%
  Most common cell type is 2 MET:2 CEP7 found in 23.33% of nuclei.
  Second most common is 4 MET:4 CEP7 found in 16.66% of nuclei.
 KRAS
  KRAS/cell: 2.21
  CEP12/cell: 2.26
  KRAS copy number ≥4: 1.00%
  Ratio KRAS/CEP12: 0.98
  Ratio KRAS/CEP12 ≥2: 0%
  Most common cell type is 2 KRAS:2 CEP12 found in 47.00% of nuclei.