Table 2.

Summary table of prognostic and genetic markers in serial CLL cases (time point 1)

No/low evolution of DNA methylation n = 19 (%)High evolution of DNA methylation n = 9 (%)P
Prognostic indicators
IGHV unmutated7 (37)9 (100)0.002
ZAP70 low methylation7 (37)9 (100)0.002
 High methylation heterogeneity5 (26)8 (89)0.002
Cytogenetics
 Normal karyotype4 (21)2 (22)n.s.
 del 13q1412 (63)6 (67)n.s.
 del 11q234 (21)1 (11)n.s.
 del 17p133 (16)1 (11)n.s.
 del 6q1 (5)1 (11)n.s.
 Trisomy 123 (16)1 (11)n.s.
Genetic mutations
TP533 (16)5 (56)0.03
SF3B14 (21)3 (33)n.s.
NOTCH3 (16)0n.s.
MYD883 (16)0n.s.
BRAF1 (5)2 (22)n.s.
KRAS01 (11)n.s.
Mutation clone size
 Clonal (>80%)7 (37)1 (11)n.s.
Subclonal (<80%)5 (26)6 (67)0.04
 Any11 (58)7 (78)n.s.